NMT历*的今天丨Planta:NMT评估种子萌发和活力

nmt是基因功能的活体检测技术,已被103位诺贝尔奖得主所在单位,及北大、清华、中科院使用。
nmt历*的今天
2014年02月19日,北京林业大学汪晓峰、李加国用nmt在planta 上发表了标题为the fluxes of h2o2 and o2 can be used to evaluate seed germination and vigor of caragana korshinskii的研究成果。
期刊:planta
主题:h2o2和o2的通量可以评估种子的萌发和活力
标题:the fluxes of h2o2 and o2 can be used to evaluate seed germination and vigor of caragana korshinskii
影响因子:3.347
检测指标:h2o2、o2流速
作者:北京林业大学汪晓峰、李加国
英文摘要
seed deterioration is detrimental to plant germplasm conservation, and predicting seed germination and vigor with reliability and sensitivity means is urgently needed for practical problems.
we investigated the link between hydrogen peroxide (h2o2) flux, oxygen influx and seed vigor of caragana korshinskii by the non-invasive micro-test technique (nmt). some related physiological and biochemical changes in seeds were also determined to further explain the changes in the molecular fluxes.
the results showed that there was a good linear relationship between germination and h2o2 flux, and that o2 influx was more suitable for assessing seed vigor. h2o2 flux changed relatively little initially, mainly affected by antioxidants (apx, cat and gsh) and h2o2 content; afterward, the efflux increased more and more rapidly due to high membrane permeability. with the damage of mitochondrial respiration and membrane integrity, o2 influx was gradually reduced.
we propose that monitoring h2o2 and o2 fluxes by nmt may be a reliable and sensitive method to evaluate seed germination and vigor.
中文摘要(谷歌机翻)
种子变质对植物种质的保存有害,因此迫切需要通过可靠性和敏感性手段预测种子的发芽和活力。
我们通过非损伤微测试技术(nmt)研究了过氧化氢(h2o2)通量,氧气流入和柠条锦鸡儿种子活力之间的联系。还确定了种子中一些相关的生理和生化变化,以进一步解释分子通量的变化。
结果表明,发芽率与h2o2通量之间存在良好的线性关系,而o2流入更适合评估种子活力。h2o2通量初变化相对较小,主要受抗氧化剂(apx,cat和gsh)和h2o2含量的影响;之后,由于高的膜渗透性,流出量越来越快地增加。随着线粒体呼吸和膜完整性的损害,o2流入量逐渐减少。
我们建议通过nmt监测h2o2和o2通量可能是评估种子发芽和活力的可靠且敏感的方法。
fig. 3 effect of artificial aging on fluxes of oxygen and h2o2. the colored lines represent the fluxes of oxygen and h2o2 over time for one seed (a, b, d, e), and each column gives the mean ± sd from ten different seeds (n = 10) (c, f). statistical analysis was done using duncan’s multiple range test. treatments with different letters are significantly different at the p < 0.05 level

高清卡口应用系统(机、非、行人混合车道模式)
粗粮食品顺应健康潮流 生产工艺是品质关键
XB型旋转式滗水器的性能特点
红色阻燃陶瓷纤维灭火毯解析
福建美国海运时效(福建美国海运)
NMT历*的今天丨Planta:NMT评估种子萌发和活力
波纹管补偿器在热力管网中的具体应用
samson定位器与执行器的安装使用说明
出口货物收取人民币货款能退税吗?(货物出口了收人民币能不能退税)
钢制拖链运行解决方案
生产B2级橡塑保温板厂家
炭黑成分的多功能性
国际航空运输解读九大航权
货代遇到的问题有哪些
气浮机适用什么类型废水
11月18日截止征意→东莞《生活垃圾焚烧炉渣综合利用工程技术规范》
加热磁力搅拌器的主要设计原理分享
飞灰固化设备固化车间除尘设备
散装机输送水泥卸料收尘伸缩布袋
贸发局是干什么的有什么用呢