anti-cd4/fitc
from:abcam
catalog number :rs-0766r-fitc
quantity size : 0.1ml(dilute with ph 7.4 0.01 m pbs or antibody diluent )background:
this gene encodes a membraneglycoprotein of t lymphocytes that interacts with major histocompatibilitycomplex class ii antigenes and is also a receptor for the humanimmunodeficiency virus. this gene is expressed not only in t lymphocytes, butalso in b cells, macrophages, and granulocytes. it is also expressed inspecific regions of the brain. the protein functions to initiate or augment theearly phase of t-cell activation, and may function as an important mediator ofindirect neuronal damage in infectious and immune-mediated diseases of thecentral nervous system. multiple alternatively spliced transcript variantsencoding different isoforms have been identified in this gene. [provided byrefseq, aug 2010].function : accessory protein for mhc class-ii antigen/t-cellreceptor interaction. may regulate t-cell activation. induces the aggregationof lipid rafts.
subunit : associates with lck. bindsto hiv-1 gp120 and to p4hb/pdi and upon hiv-1 binding to the cell membrane, ispart of p4hb/pdi-cd4-cxcr4-gp120 complex. interacts with hiv-1 envelopepolyprotein gp160 and protein vpu. interacts with human herpes virus 7 capsidproteins. interacts with ptk2/fak1; this interaction requires the presence ofhiv-1 gp120.subcellular location : cell membrane; single-pass type i membraneprotein. note=localizes to lipid rafts. removed from plasma membrane by hiv-1nef protein that increases clathrin-dependent endocytosis of this antigen totarget it to lysosomal degradation. specificity :anti-cd4/fitc is a mousemonoclonall antibody
specific for cd4/fitc ofhuman,rb
–use for western blotting,elisa,immunoprecipitationand immunohistochemistry
–protein g affinity chromatographypurification, purity :>95%
–isotype: igg
–mol wt:48kda
application :
–flow-cyt=1:50-200if=1:50-200
–excitation spectrum:520-570nm
–emission spectrum:490-510nm
–not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
storage: storeat -20℃ for oneyear. avoid repeated freeze/thaw cycles. thelyophilized antibody is stable at room temperature for at least onemonth and for greater than a year whenkept at -20℃.when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for atleast six weeks at 2-4 ℃
important note: thisproduct as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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