Epicypher：EpiDyne-FRET Nucleosome Remodeling Assay Substrate
当与酶活性重塑复合物如smarca4、smarca2和acf（如epicypher货号15-1014、15-1015、15-1013）配对时，该产品提供核小体重塑的高通量荧光读数。epidyne®-fret单核小体由在大肠杆菌中表达的重组人组蛋白（组蛋白h2a-cy5、h2b、h3.2和h4各两种；accession numbers:：h2a-p4908；h2b-o60814；h3.2-q71di3；h4-p62805）组装而成，该组蛋白由212个dna碱基对缠绕，并携带5'cy3、widom 601核小体定位元件和重塑时暴露的dpnii限制性内切酶基序（gatc）[1]。h2a在残基120位处具有thr-cys取代，其中cy5是共轭的。h3.2在残基110位处具有cys-to-ala取代。
[1] lowary & widom j mol. biol. (1998) pmid: 9514715.
产品详情
保存温度:stable for six (6) months at -80°c from date of receipt. for best results, aliquot and avoid multiple freeze/thaws.
运输温度:dry ice.
产品形式:purified recombinant mononucleosomes, (21.8 µg protein weight, 50 µg dna+protein) in 44.2 µl of 10 mm tris-hcl ph 7.5, 25 mm nacl, 1 mm edta, 2 mm dtt, 20% glycerol. mw = 240,650 da. molarity = 4.7 µm.
验证数据
figure 1: dna gel data
epidyne-fret substrate resolved via native page gel and
stained with ethidiumbromide to visualize dna. lane 1: free
dna (100 ng). lane 2: intact epidyne-fretnucleosomes
(400 ng).
figure 2: protein gel data
coomassie stained page gel of proteins in epidyne-fret
substrate (1 µg)demonstrates the purity of histones in the
preparation. sizes of molecularweight markers and positions
of the core histones (h2a-cy5, h2b, h3.2, and h4)are
indicated.
figure 3: nucleosome remodeling data
acf1/atp-dependent nucleosome remodeling reaction. epidyne-fret
nucleosomes (15 nm) wereincubated with acf1 chromatin remodeler
(epicypher 15-1013; indicated concentrations) in the presenceor absence
of 1 mm atp. upon the addition of atp, reactions were immediately read
in an envision multilabelplate reader (perkinelmer). data are presented
as the mean of the cy3/cy5 ratio (n=2).
订购详情
货号
产品名称
规格
16-4201
epidyne-fret nucleosome remodeling assay substrate
50 µg
如需了解更多详细信息或相关产品，请联系epicypher中国授权代理商-欣博盛生物
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[1] lowary & widom j mol. biol. (1998) pmid: 9514715.
产品详情
保存温度:stable for six (6) months at -80°c from date of receipt. for best results, aliquot and avoid multiple freeze/thaws.
运输温度:dry ice.
产品形式:purified recombinant mononucleosomes, (21.8 µg protein weight, 50 µg dna+protein) in 44.2 µl of 10 mm tris-hcl ph 7.5, 25 mm nacl, 1 mm edta, 2 mm dtt, 20% glycerol. mw = 240,650 da. molarity = 4.7 µm.
验证数据
figure 1: dna gel data
epidyne-fret substrate resolved via native page gel and
stained with ethidiumbromide to visualize dna. lane 1: free
dna (100 ng). lane 2: intact epidyne-fretnucleosomes
(400 ng).
figure 2: protein gel data
coomassie stained page gel of proteins in epidyne-fret
substrate (1 µg)demonstrates the purity of histones in the
preparation. sizes of molecularweight markers and positions
of the core histones (h2a-cy5, h2b, h3.2, and h4)are
indicated.
figure 3: nucleosome remodeling data
acf1/atp-dependent nucleosome remodeling reaction. epidyne-fret
nucleosomes (15 nm) wereincubated with acf1 chromatin remodeler
(epicypher 15-1013; indicated concentrations) in the presenceor absence
of 1 mm atp. upon the addition of atp, reactions were immediately read
in an envision multilabelplate reader (perkinelmer). data are presented
as the mean of the cy3/cy5 ratio (n=2).
订购详情
货号
产品名称
规格
16-4201
epidyne-fret nucleosome remodeling assay substrate
50 µg
如需了解更多详细信息或相关产品，请联系epicypher中国授权代理商-欣博盛生物
如何评价海外仓储？
保温管补口就用电热熔套-高尔定做热熔套厂家
畜肉或水产品变质检测设备是如何检测肉类产品的
中国和美国海运线路一样吗（美国中国海运路线图）
又有两家船公司，豪发巨额年终奖！天价年终奖还能发多久？
Epicypher：EpiDyne-FRET Nucleosome Remodeling Assay Substrate
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使用人员需要了解的运行中的德国HAWE截止阀G4-1R-G205型维护手册
气相色谱仪受捧它有哪些特点？
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