dna损伤酶联免疫检测试剂盒dna damage elisa kit—enzo life sciences热销产品
——用于肿瘤、细胞凋亡和氧化应激研究中dna损伤的快速检测
细胞暴露于氧化和环境应激经常导致基因组dna的分解或氧化,评价基因组dna完整性或评估氧化dna存在的测定法经常用作验证凋亡或dna损伤开始的手段。8-羟基-2 '-脱氧鸟苷(8-ohdg)是一种修饰的核苷碱基,是氧化dna损伤和氧化应激的常用生物标志物。尿液中的8-ohdg及其类似物8-羟基鸟苷和8-羟基鸟嘌呤与许多退行性疾病有关。在许多疾病中,包括膀胱癌和前列腺癌,囊性纤维化,特应性皮炎和类风湿性关节炎中,已经研究了ros的相关性和使用8-ohdg作为氧化应激的生物标志物。帕金森氏病、阿尔茨海默氏病和亨廷顿氏病是神经退行性疾病,被认为是由具有这些疾病遗传倾向的人接触神经毒素引起的。氧化应激与这些疾病的发病机制有关,在多种神经系统疾病中检测到dna损伤水平升高。
enzo life sciences的dna损伤酶联免疫检测试剂盒是一种快速灵敏的竞争法免疫检测试剂盒,可用于检测和定量尿液、血清和唾液样品中的8-羟基-2 '-脱氧鸟苷(8- ohdg)。
产品特点
● 高灵敏度,可检测低至1 ng/ml 8-ohdg
●在多类型样本中验证并获得可靠结果
●优于半定量western blot分析的全定量检测
●无边缘效应,每个试剂盒可检测更多样本
产品详情
产品货号
adi-eks-350
产品名称
dna damage elisa kit(dna损伤elisa试剂盒)
规格
96 wells
灵敏度
0.59 ng/ml
检测范围
0.94 - 60 ng/ml
检测时间
<2.5 hours
适用样本类型
血清,唾液,尿液(有文献报道血浆、精液、细胞裂解物、培养上清液和dna提取物)
适用种属
不限物种
交叉反应
the dna damage elisa also detects 8-hydroxyguanosine (product of oxidative rna damage)
and 8-hydroxyguanine (product of oxidative dna damage by hydroxyl radicals)
试剂盒组分
microtiter plate, standard, sample diluent, wash buffer concentrate, antibody, antibody diluent,
conjugate, conjugate diluent, tmb substrate, stop solution 2
标准曲线示例
部分产品文献引用
1. the dietary fermentable fiber inulin alters the intestinal microbiome and improves chronickidney disease mineral-bone disorder in a rat model of ckd: a. biruete, et al.; biorxiv (2023)
2. octanoic acid - an insecticidal metabolite of conidiobolus coronatus (entomopthorales) that affects two majors antifungal protection systems in galleria mellonella (lepidoptera): cuticular lipids and hemocyte: a. kaczmarek, et al.; int. j. mol. sci. 23, 5204 (2022)
3. wasp venom ameliorates scopolamine-induced learning and memory impairment in mice: j.h. chae, et al.; toxins 14, 256 (2022)
4. arbutin protects brain against middle cerebral artery occlusion-reperfusion (mcao/r) injury: m. kumar, et al.; biochem. biophys. res. commun. 577, 52 (2021)
5. berberine ameliorates renal injury in a rat model of d-galactose-induced aging through a pten/akt-dependent mechanism: h.e. el-horany, et al.; arch. physiol. biochem. 126, 157 (2020), application(s): rat blood serum
6. evaluation of effectiveness of ultraviolet emitting lamps on the cutaneous production of vitamin d3: relationship of the lamps vitamin d3 producing potential to the production of 8-hydroxy-2'-deoxyguanosine and nitric oxide: a. shirvani, et al.; anticancer res. 40, 565 (2020)
7. in vivo anti-inflammatory potential of viscozyme®-treated jujube fruit: y. kim, et al.; foods 9, 1033 (2020), application(s): mouse liver tissue homogenate
8. inhibitory effect of steamed soybean wastewater against dss-induced intestinal inflammation in mice: s. jeong, et al.; foods 9, 954 (2020), application(s): quantification of 8-ohdg level in mouse plasma.
9. n-acetylcysteine (nac), an anti-oxidant, does not improve bone mechanical properties in a rat model of progressive chronic kidney disease-mineral bone disorder: m.r. allen, et al.; plos one 15, e0230379 (2020)
10. neuroprotective effects of euonymus alatus extract on scopolamine-induced memory deficits in mice: y. woo, et al.; antioxidants (basel) 9, 449 (2020), application(s): serum
11. antioxidative effects of olive polyphenols compared to vitamin e in piglets fed a diet rich in n-3 pufa: j. leskovec, et al.; animals (basel) 9, 161 (2019), application(s): elisa using pig serum
12. microrna-204-3p attenuates high glucose-induced mpc5 podocytes apoptosis by targeting braykinin b2 receptor: x. han, et al.; exp. clin. endocrinol. diabetes 127, 387 (2019), application(s): mouse mpc5 podocyte lysates
13. oxidative stress and mitochondrial responses to stress exposure suggest that king penguins are naturally equipped to resist stress: a. stier, et al.; sci. rep. 9, 8545 (2019), application(s): penguin plasma and red blood cell lysate
详情请联系enzo life sciences中国授权代理-欣博盛生物
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Enzo Life Sciences DNA损伤酶联免疫检测试剂盒DNA damage ELISA kit
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