rabbitanti-wnt3a
catalognumber:hz94876quantitysize:0.1ml(dilutewithph7.40.01mpbsorantibodydiluent)
background:wnt3aisasecretedligandformembersofthefrizzledfamilyofreceptors.itisoneoftheclosely-relatedwntfamilyandhasmultiplerolesindevelopmentandcellfate.wnt3ashowsparticularsequenceidentitywithwnt3despitetheirdivergentroles.wnt3isrequiredfornormallimbdevelopmentandplaysanimportantroleintheestablishmentoftheapicalectodermalridge.inmotorneurons,wnt3isaretrogradesignalthatcontrolsterminalbranchingofmuscleafferents.wnt3ishighlyexpressedinhumancancercelllinesincludinga549(lungcancer),mkn45(gastriccancer)andmcf7(breastcancer).
specificity:
anti-wnt3aisarabbitpolyclonalantibodyunconjugated specificforwnt3aofhuman,mouseandrat useforwesternblotting,elisa,immunoprecipitationandimmunohistochemistry proteingaffinitychromatographypurification,purity:>95% isotype:igg molwt:37kdaapplication:
westernblotting1:100-500 immunohistochemistry1:100-500 elisa1:500-1000 optimalworkingdilutionsmustbedeterminedbytheenduser.storage:storeat-20℃foroneyear.avoidrepeatedfreeze/thawcycles.thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20℃.whenreconstitutedinsterileph7.40.01mpbsordiluentofantibody,theantibodyisstableforatleastsixweeksat2-4℃
importantnote:thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
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