吸入TiO2纳米结构气溶胶大鼠支气管肺泡灌洗液的蛋白质组学分析
proteomic analysis of bronchoalveolar lavage fluid in rat exposed to tio2 nanostructured aerosol by inhalation
吸入tio2纳米结构气溶胶大鼠支气管肺泡灌洗液的蛋白质组学分析
laëtitia chézeaua,b, lori a. kohlstaedtc, alain le faoub, frédéric cosniera, bertrand rihnb,d, laurent gatéa,?
a institut national de recherche et de sécurité, rue du morvan, cs 60027,vandoeuvre, cedex, france
b ea 3452 cithefor, université de lorraine, bp 80403,nancy cedex, france
c california institute for quantitative biosciences, university of california, berkeley, ca 94720, united states of america
d institut jean-lamour, umr 7198 cnrs, université de lorraine,nancy cedex, france
a b s t r a c t
the pulmonary toxicological properties of inhaled titanium dioxide were studied using bronchoalveolar lavage fluid (balf) cytology and proteomics analyses. fischer 344 rats were exposed to 10 mg/m3 of tio2 nanostructured aerosol by nose-only inhalation for 6 h/day, 5 days/week for 4 weeks. lung samples were collected up to 180 post-exposure days. as previously described, cytological analyses of balf showed a strong inflammatory response up to 3 post-exposure days, which persisted however, at a lower intensity up to 180 days. in addition, using multidimensional protein identification technology (mudpit), we identified a total of 107, 50 and 45 proteins (uniprotkb identifiers) differentially expressed in exposed rats immediay, 3 and 180 days after the end of exposure respectively. increased levels of inflammatory proteins, members of proteasome, various histones, proteins involved in cytoskeleton organization, were noticed up to 3 days (short-term response). some of these proteins were linked with neutrophil extracellular trap formation (netosis). long-term response was also characterized by a persistent altered expression of proteins up to 180 days. altogether, these results suggest that exposure to low toxicity low solubility nanomaterials such as tio2 may induce long-term changes in the pulmonary protein expression pattern of which the physio-pathological consequences are unknown. significance: this paper describes in rats, at the pulmonary level, the effects of inhaled nanostructured aerosol of tio2 on the secreted proteins found in the broncho-alveolar space by comparing the proteomic profile in broncho-alveolar lavage fluid supernatants of control and exposed animals. this work brings new insights about the early events occurring following the end of exposure and suggests the formation of neutrophil extracellular traps (netosis) that could be interpret as a potential early mechanism of defense against tio2 nanoparticles. this work also describes the long term effects (180 post-exposure days) of such an exposure and the change in secreted protein expression in the absence of significant histopathological modifications.
用支气管肺泡灌洗液(balf)细胞学和蛋白质组学分析研究吸入*的肺毒理特性。fischer 344大鼠仅经鼻吸入10mg/m3 tio2纳米结构气雾剂6小时/天，5天/周，连续4周。在暴露后180天内采集肺样本。如前所述，balf的细胞学分析显示，暴露后3天内有强烈的炎症反应，但持续时间较低，达180天。此外，利用多维蛋白质鉴定技术(mudpit)，我们分别鉴定了暴露后即刻、3天和180天暴露大鼠体内差异表达的107、50和45种蛋白质(uniprotkb标识符)。炎性蛋白、蛋白酶体成员、各种组蛋白、参与细胞骨架组织的蛋白水平增加，长3天(短期反应)。其中一些蛋白与中性粒细胞胞外陷阱形成(netosis)有关。长期反应还表现为持续180天的蛋白质表达改变。总之，这些结果表明，暴露于低毒性的低溶解度纳米材料如tio2可诱导肺组织蛋白表达模式的长期变化，其中生理病理后果是未知的。意义：通过比较对照组和暴露组大鼠支气管肺泡灌洗液上清液的蛋白质组学特征，本文在大鼠肺水平上研究了吸入*纳米结构气雾剂对支气管肺泡腔分泌蛋白的影响。这项工作为暴露结束后发生的早期事件带来了新的见解，并提出了中性粒细胞胞外陷阱(netosis)的形成，可以解释为对tio2纳米颗粒防御的潜在早期机制。这项工作还描述了这种暴露的长期影响(暴露后180天)以及在没有明显组织病理学改变的情况下分泌蛋白表达的变化。
keywords: titanium dioxide、lung inflammation、protein expression profile、short-term response、long-term response、netosis
关键词：*、肺部炎症、蛋白表达谱、短期反应、长期反应、网状内皮细胞增生
简易手推龙门架
LL-275-133美国NOR-CAL泄压阀工作原理
光合作用检测仪测定方法
陕西省DP电缆西门子*总代理商
苏州赛普介绍塑料科斯特花环填料在煤气净化塔的应用
吸入TiO2纳米结构气溶胶大鼠支气管肺泡灌洗液的蛋白质组学分析
概述硫酸流量计的选用及安装方法
蔬菜脱水机的注意事项有哪些
实时荧光定量PCR系统知识合集
如何保养三次元测量仪传动部分
BC-160水泥比长仪构成量程
污水处理效果与温度的关系
通风设备有哪些？有什么作用？
卧式燃油蒸汽锅炉有哪些安全性能
柔性难燃橡塑保温板厂家
如何正确又*的使用旋耕机
摇臂钻床的运动
刮板输送机的用途!
开盘门禁摇号系统方案
蒸馏塔设计方案解读
吸入tio2纳米结构气溶胶大鼠支气管肺泡灌洗液的蛋白质组学分析
laëtitia chézeaua,b, lori a. kohlstaedtc, alain le faoub, frédéric cosniera, bertrand rihnb,d, laurent gatéa,?
a institut national de recherche et de sécurité, rue du morvan, cs 60027,vandoeuvre, cedex, france
b ea 3452 cithefor, université de lorraine, bp 80403,nancy cedex, france
c california institute for quantitative biosciences, university of california, berkeley, ca 94720, united states of america
d institut jean-lamour, umr 7198 cnrs, université de lorraine,nancy cedex, france
a b s t r a c t
the pulmonary toxicological properties of inhaled titanium dioxide were studied using bronchoalveolar lavage fluid (balf) cytology and proteomics analyses. fischer 344 rats were exposed to 10 mg/m3 of tio2 nanostructured aerosol by nose-only inhalation for 6 h/day, 5 days/week for 4 weeks. lung samples were collected up to 180 post-exposure days. as previously described, cytological analyses of balf showed a strong inflammatory response up to 3 post-exposure days, which persisted however, at a lower intensity up to 180 days. in addition, using multidimensional protein identification technology (mudpit), we identified a total of 107, 50 and 45 proteins (uniprotkb identifiers) differentially expressed in exposed rats immediay, 3 and 180 days after the end of exposure respectively. increased levels of inflammatory proteins, members of proteasome, various histones, proteins involved in cytoskeleton organization, were noticed up to 3 days (short-term response). some of these proteins were linked with neutrophil extracellular trap formation (netosis). long-term response was also characterized by a persistent altered expression of proteins up to 180 days. altogether, these results suggest that exposure to low toxicity low solubility nanomaterials such as tio2 may induce long-term changes in the pulmonary protein expression pattern of which the physio-pathological consequences are unknown. significance: this paper describes in rats, at the pulmonary level, the effects of inhaled nanostructured aerosol of tio2 on the secreted proteins found in the broncho-alveolar space by comparing the proteomic profile in broncho-alveolar lavage fluid supernatants of control and exposed animals. this work brings new insights about the early events occurring following the end of exposure and suggests the formation of neutrophil extracellular traps (netosis) that could be interpret as a potential early mechanism of defense against tio2 nanoparticles. this work also describes the long term effects (180 post-exposure days) of such an exposure and the change in secreted protein expression in the absence of significant histopathological modifications.
用支气管肺泡灌洗液(balf)细胞学和蛋白质组学分析研究吸入*的肺毒理特性。fischer 344大鼠仅经鼻吸入10mg/m3 tio2纳米结构气雾剂6小时/天，5天/周，连续4周。在暴露后180天内采集肺样本。如前所述，balf的细胞学分析显示，暴露后3天内有强烈的炎症反应，但持续时间较低，达180天。此外，利用多维蛋白质鉴定技术(mudpit)，我们分别鉴定了暴露后即刻、3天和180天暴露大鼠体内差异表达的107、50和45种蛋白质(uniprotkb标识符)。炎性蛋白、蛋白酶体成员、各种组蛋白、参与细胞骨架组织的蛋白水平增加，长3天(短期反应)。其中一些蛋白与中性粒细胞胞外陷阱形成(netosis)有关。长期反应还表现为持续180天的蛋白质表达改变。总之，这些结果表明，暴露于低毒性的低溶解度纳米材料如tio2可诱导肺组织蛋白表达模式的长期变化，其中生理病理后果是未知的。意义：通过比较对照组和暴露组大鼠支气管肺泡灌洗液上清液的蛋白质组学特征，本文在大鼠肺水平上研究了吸入*纳米结构气雾剂对支气管肺泡腔分泌蛋白的影响。这项工作为暴露结束后发生的早期事件带来了新的见解，并提出了中性粒细胞胞外陷阱(netosis)的形成，可以解释为对tio2纳米颗粒防御的潜在早期机制。这项工作还描述了这种暴露的长期影响(暴露后180天)以及在没有明显组织病理学改变的情况下分泌蛋白表达的变化。
keywords: titanium dioxide、lung inflammation、protein expression profile、short-term response、long-term response、netosis
关键词：*、肺部炎症、蛋白表达谱、短期反应、长期反应、网状内皮细胞增生
简易手推龙门架
LL-275-133美国NOR-CAL泄压阀工作原理
光合作用检测仪测定方法
陕西省DP电缆西门子*总代理商
苏州赛普介绍塑料科斯特花环填料在煤气净化塔的应用
吸入TiO2纳米结构气溶胶大鼠支气管肺泡灌洗液的蛋白质组学分析
概述硫酸流量计的选用及安装方法
蔬菜脱水机的注意事项有哪些
实时荧光定量PCR系统知识合集
如何保养三次元测量仪传动部分
BC-160水泥比长仪构成量程
污水处理效果与温度的关系
通风设备有哪些？有什么作用？
卧式燃油蒸汽锅炉有哪些安全性能
柔性难燃橡塑保温板厂家
如何正确又*的使用旋耕机
摇臂钻床的运动
刮板输送机的用途!
开盘门禁摇号系统方案
蒸馏塔设计方案解读