Plant Sci：湖南农大丨氮素利用效率与镉耐受性的平衡
期刊：plant science
主题：氮素利用效率与镉耐受性的平衡
标题：balance between nitrogen use efficiency and cadmium tolerance in brassica napus and arabidopsis thaliana
影响因子：3.785
检测指标： h+、cd2+、no3-流速
检测部位：根部液泡
h+、cd2+、no3-流实验处理方法：
4周龄拟南芥，20 μm cdcl2处理3天
21日龄油菜，100 μm cdcl2处理7天
h+、cd2+、no3-流实验测试液成份：
文献无
*测试液成份：
拟南芥根部液泡：0.002mm cdcl2，0.1 mm kno3 , 600 mm mannitol, 0.05mm mes, ph 7.2
油菜根部液泡：0.01mm cdcl2，0.1 mm kno3 , 600 mm mannitol, 0.05mm mes, ph 7.2
作者：湖南农业大学张振华、廖琼
英文摘要
the transmembrane transport of no3− andcd2+ into plant cell vacuoles relies on the energy from their tonoplast protonpumps, v-atpase and v-ppase. if the activity of these pumps is reduced, itresults in less no3− and cd2+ being transported into the vacuoles, whichcontributes to better nitrogen use efficiency (nue) and lower cd2+ tolerance inplants.
the physiological mechanisms that regulatethe balance between nue and cd2+ tolerance remain unknown. in our study, twobrassica napus genotypes with differential nues, xiangyou 15 and 814, andatclca-2 mutant and atcax4 over-expression line (atcax4-oe) of arabidopsisthaliana, were used to investigate cd2+ stress responses.
we found that the brassica napus genotype,with higher nue, was more sensitive to cd2+ stress. the atcax4-oe mutant, withhigher cd2+ vacuolar sequestration capacity (vsc), limited no3− sequestrationinto root vacuoles and promoted nue. atclca-2 mutants, with decreased no3− vsc,enhanced cd2+ sequestration into root vacuoles and conferred greater cd2+tolerance than the wt. this may be due to the competition between cd2+ andno3−in the vacuoles for the energy provided by v-atpase and v-ppase. regulating thebalance between cd2+ and no3− vacuolar accumulation by inhibiting the activityof clca transporter and increasing the activity of cax4 transporter willsimultaneously enhance both the nue and cd2+ tolerance of brassica napus,essential for improving its cd2+ phytoremediation potential.
中文摘要（谷歌机翻）
no3-和cd2+跨膜转运到植物细胞液泡中依赖于其液泡膜质子泵，v-atp酶和v-ppase的能量。如果这些泵的活性降低，则导致较少的no3-和cd2+被输送到液泡中，这有助于提高植物的氮利用效率（nue）和降低cd2+耐受性。
调节nue和cd2+耐受性之间平衡的生理机制尚不清楚。在我们的研究中，使用具有差异nue，香优15和814的两种甘蓝型油菜基因型和拟南芥的atclca-2突变体和atcax4过表达系（atcax4-oe）来研究cd2+应激反应。
我们发现甘蓝型油菜基因型具有较高的nue，对cd2+胁迫更敏感。具有较高cd2+液泡隔离能力（vsc）的atcax4-oe突变体限制no3-隔离到根空泡中并促进nue。具有降低的no3-vsc的atclca-2突变体增强了cd2+隔离到根空泡中并且赋予比wt更大的cd2+耐受性。这可能是由于v-atp酶和v-ppase提供的能量在液泡中cd2+和no3-之间的竞争。通过抑制clca转运蛋白的活性和增加cax4转运蛋白的活性来调节cd2+和no3-液泡积累之间的平衡将同时增强甘蓝型油菜的nue和cd2+耐受性，这对于改善其cd2+植物修复潜力是必需的。
fig. 2. the arabidopsis thaliana vha-a2, vha-a3, and avp1 mutants were more sensitive to cd2+ stress than the wild-type col-0. (a) the phenotype and (b) the chlorophyll loss, in relation to the controls, of col-0 and the vha-a2, vha-a3, and avp1 mutants grown for 4 weeks and then exposed to 20 μm cdcl2 for 3 days. mean rates of (c) h+ fluxes and (d) cd2+ fluxes during the first 160 s of measurements within the root vacuoles.
高低温试验机冷系统维修中的注意事项
如何维持自动滚齿机的加工精度
油浸式变压器需要按时加油吗？
氧化锌避雷器测试仪制造商为您讲述我国仪器仪表业亟待产业升级
2814508 MCR3路隔离放大器用于模拟信号的电隔离
Plant Sci：湖南农大丨氮素利用效率与镉耐受性的平衡
了解防火橡塑保温管施工图
夹层煮锅的使用注意事项
药用级糊精药典备案状态A
辐射照度测量仪的选购
上海东麓热空气消毒箱的结构概述
制药厂空气湿度超标怎么办？制药厂除湿机
PILAN管壳式换热器 TP-A2特征及优点介绍
ATOS放大器E-MEAC-05F基本结构和主要原理
弹簧管压力表回程误差超差怎么办?
伯朗特机器人供应商带你认识所有机器人
青岛数控冲床多少钱一台
超声波探伤仪的主要特性及优点
5吨商业食物湿垃圾处理器方案
玻璃瓶垂直偏差的检测意义
主题：氮素利用效率与镉耐受性的平衡
标题：balance between nitrogen use efficiency and cadmium tolerance in brassica napus and arabidopsis thaliana
影响因子：3.785
检测指标： h+、cd2+、no3-流速
检测部位：根部液泡
h+、cd2+、no3-流实验处理方法：
4周龄拟南芥，20 μm cdcl2处理3天
21日龄油菜，100 μm cdcl2处理7天
h+、cd2+、no3-流实验测试液成份：
文献无
*测试液成份：
拟南芥根部液泡：0.002mm cdcl2，0.1 mm kno3 , 600 mm mannitol, 0.05mm mes, ph 7.2
油菜根部液泡：0.01mm cdcl2，0.1 mm kno3 , 600 mm mannitol, 0.05mm mes, ph 7.2
作者：湖南农业大学张振华、廖琼
英文摘要
the transmembrane transport of no3− andcd2+ into plant cell vacuoles relies on the energy from their tonoplast protonpumps, v-atpase and v-ppase. if the activity of these pumps is reduced, itresults in less no3− and cd2+ being transported into the vacuoles, whichcontributes to better nitrogen use efficiency (nue) and lower cd2+ tolerance inplants.
the physiological mechanisms that regulatethe balance between nue and cd2+ tolerance remain unknown. in our study, twobrassica napus genotypes with differential nues, xiangyou 15 and 814, andatclca-2 mutant and atcax4 over-expression line (atcax4-oe) of arabidopsisthaliana, were used to investigate cd2+ stress responses.
we found that the brassica napus genotype,with higher nue, was more sensitive to cd2+ stress. the atcax4-oe mutant, withhigher cd2+ vacuolar sequestration capacity (vsc), limited no3− sequestrationinto root vacuoles and promoted nue. atclca-2 mutants, with decreased no3− vsc,enhanced cd2+ sequestration into root vacuoles and conferred greater cd2+tolerance than the wt. this may be due to the competition between cd2+ andno3−in the vacuoles for the energy provided by v-atpase and v-ppase. regulating thebalance between cd2+ and no3− vacuolar accumulation by inhibiting the activityof clca transporter and increasing the activity of cax4 transporter willsimultaneously enhance both the nue and cd2+ tolerance of brassica napus,essential for improving its cd2+ phytoremediation potential.
中文摘要（谷歌机翻）
no3-和cd2+跨膜转运到植物细胞液泡中依赖于其液泡膜质子泵，v-atp酶和v-ppase的能量。如果这些泵的活性降低，则导致较少的no3-和cd2+被输送到液泡中，这有助于提高植物的氮利用效率（nue）和降低cd2+耐受性。
调节nue和cd2+耐受性之间平衡的生理机制尚不清楚。在我们的研究中，使用具有差异nue，香优15和814的两种甘蓝型油菜基因型和拟南芥的atclca-2突变体和atcax4过表达系（atcax4-oe）来研究cd2+应激反应。
我们发现甘蓝型油菜基因型具有较高的nue，对cd2+胁迫更敏感。具有较高cd2+液泡隔离能力（vsc）的atcax4-oe突变体限制no3-隔离到根空泡中并促进nue。具有降低的no3-vsc的atclca-2突变体增强了cd2+隔离到根空泡中并且赋予比wt更大的cd2+耐受性。这可能是由于v-atp酶和v-ppase提供的能量在液泡中cd2+和no3-之间的竞争。通过抑制clca转运蛋白的活性和增加cax4转运蛋白的活性来调节cd2+和no3-液泡积累之间的平衡将同时增强甘蓝型油菜的nue和cd2+耐受性，这对于改善其cd2+植物修复潜力是必需的。
fig. 2. the arabidopsis thaliana vha-a2, vha-a3, and avp1 mutants were more sensitive to cd2+ stress than the wild-type col-0. (a) the phenotype and (b) the chlorophyll loss, in relation to the controls, of col-0 and the vha-a2, vha-a3, and avp1 mutants grown for 4 weeks and then exposed to 20 μm cdcl2 for 3 days. mean rates of (c) h+ fluxes and (d) cd2+ fluxes during the first 160 s of measurements within the root vacuoles.
高低温试验机冷系统维修中的注意事项
如何维持自动滚齿机的加工精度
油浸式变压器需要按时加油吗？
氧化锌避雷器测试仪制造商为您讲述我国仪器仪表业亟待产业升级
2814508 MCR3路隔离放大器用于模拟信号的电隔离
Plant Sci：湖南农大丨氮素利用效率与镉耐受性的平衡
了解防火橡塑保温管施工图
夹层煮锅的使用注意事项
药用级糊精药典备案状态A
辐射照度测量仪的选购
上海东麓热空气消毒箱的结构概述
制药厂空气湿度超标怎么办？制药厂除湿机
PILAN管壳式换热器 TP-A2特征及优点介绍
ATOS放大器E-MEAC-05F基本结构和主要原理
弹簧管压力表回程误差超差怎么办?
伯朗特机器人供应商带你认识所有机器人
青岛数控冲床多少钱一台
超声波探伤仪的主要特性及优点
5吨商业食物湿垃圾处理器方案
玻璃瓶垂直偏差的检测意义