线粒体膜电位细胞毒性检测试剂盒--ENZO LIFE SCIENCE热销产品
enzo life sciences的mito-id®membrane potential cytotoxicity kit利用阳离子双发射染料检测线粒体膜电位（mmp）的波动，该染料在细胞质中以绿色荧光单体的形式存在，在线粒体中以橙色荧光j聚集体的形式聚集。具有低膜电位的线粒体将积累低浓度的染料并呈现绿色荧光，而更高度极化的线粒体将呈现橙红色荧光。随着线粒体功能受损加剧，细胞表现出从橙色荧光到绿色荧光的转变。该试剂盒是一种独-特的hts测定法，无需洗涤或去除培养基即可实时监测线粒体膜电位。
产品特点
●灵敏度是jc-1荧光染料的10倍，并具有卓-越的水溶性
●具备光稳定性的双发射染料
●无需漂洗/换液步骤
●单独的mito-id®检测可用于检测线粒体质量
●可在较低的药物/剂量浓度下检测细胞毒性
●没有使用jc-1染料时出现的溶剂伪影
●适用于高通量应用
实验示例
图1.检测线粒体紊乱的灵敏度是jc-1的10倍。使用mito-id®膜电位染料（红色）或jc-1（蓝色）在经cccp处理的hela细胞中评估线粒体膜电位（mmp）。使用传统的荧光酶标仪检测，结果显示mmp随着cccp浓度的增加而减少，橙色荧光减少。染料的水溶性优化和无需洗涤的实验方案最大限度减小了可变性，因此z因子（>0.9）高于使用jc-1.
图2.在药物筛选中实时检测有丝分裂毒性。使用biotek synergy™ mx荧光酶标仪对线粒体膜电位变化的时间进程研究。hela细胞与mito-id®膜电位染料在室温下孵育30分钟（不去除血清或培养基）。添加鱼藤酮分别达到1µm、3µm和9µm的浓度。橙色信号的减少证明染料对鱼藤酮有反应。
产品信息
产品货号
enz-51019-kp002
产品名称
mito-id®membrane potential cytotoxicity kit
规格
1 kit
短期保存
-20°c
长期保存
-80°c
试剂盒组分
mito-id®mp detection reagent, 200 μl
cccp control, 100 μl
10x assay buffer 1: 2.5 ml
50x assay buffer 2: 0.5 ml
应用
htsmicroplate
部分产品引用文献
1. novel silver complexes based on phosphanes and ester derivatives of bis(pyrazol-1-yl)acetate ligands targeting trxr: new promising chemotherapeutic tools relevant to sclc management: m. pellei, et al.; int. j. mol. sci.24, 4091 (2023)
2. fundc1 regulates receptor-mediated mitophagy independently of the pink1/parkin-dependent pathway in rotenone-treated sh-sy5y cells: s.y. park, et al.; food chem. toxicol.137, 111163 (2020),application(s):fluorescence microscopy and microplate reader
3. inhibitory role of trip-br1/xiap in necroptosis under nutrient/serum starvation: z. sandag, et al.; mol. cells43, 236 (2020)
4. nad hydrolysis by the tuberculosis necrotizing toxin induces lethal oxidative stress in macrophages: d. pajuelo, et al.; cell. microbiol.22, e13115 (2020),application(s):thp-1 macrophages; microplate reader
5. impaired autophagic and mitochondrial functions are partially restored by ert in gaucher and fabry diseases: m.m. ivanova, et al.; plos one14, e0210617 (2019),application(s):fluorescence microscopy
6. inhibitory role of ampactivated protein kinase in necroptosis of hct116 colon cancer cells with p53 null mutation under nutrient starvation: d.t. le, et al.; int. j. oncol.54, 702 (2019)
7. ros as a novel indicator to predict anticancer drug efficacy: t. zaidieh, et al.; bmc cancer19, 1224 (2019)
8. trem1/3 deficiency impairs tissue repair after acute kidney injury and mitochondrial metabolic flexibility in tubular epithelial cells: a. tammaro, et al.; front. immunol.10, 1469 (2019),application(s):microplate reader
9. anti-cancerous effect of cis-khellactone from angelica amurensis through the induction of three programmed cell deaths: s. jung, et al.; oncotarget9, 16744 (2018),application(s):microplate reader
10. blue light phototoxicity toward human corneal and conjunctival epithelial cells in basal and hyperosmolar conditions: v. marek, et al.; free radic. biol. med.126, 27 (2018),application(s):microplate reader
11. cgas drives noncanonical-inflammasome activation in age-related macular degeneration: n. kerur, et al.; nat. med.24, 50 (2018)
12. cytotoxicity of propofol in human induced pluripotent stem cell-derived cardiomyocytes: k. kido, et al.; j. anesth.32, 120 (2018),application(s):microplate reader
13. development of novel amino-quinoline-5, 8-dione derivatives as nad (p) h: quinone oxidoreductase 1 (nqo1) inhibitors with potent antiproliferative activities: y. ling, et al.; eur. j. med. chem.154, 199 (2018)
14. inhibition of apoptosis using exosomes in chinese hamster ovary cell culture: s. han, et al.; biotechnol. bioeng.115, 1331 (2018)
15. light action spectrum on oxidative stress and mitochondrial damage in a2e-loaded retinal pigment epithelium cells: m. marie, et al.; cell death disc.9, 287 (2018)
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线粒体膜电位细胞毒性检测试剂盒--ENZO LIFE SCIENCE热销产品
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兴港投资集团是国企吗（郑州航空港兴港投资集团有限公司简介）
产品特点
●灵敏度是jc-1荧光染料的10倍，并具有卓-越的水溶性
●具备光稳定性的双发射染料
●无需漂洗/换液步骤
●单独的mito-id®检测可用于检测线粒体质量
●可在较低的药物/剂量浓度下检测细胞毒性
●没有使用jc-1染料时出现的溶剂伪影
●适用于高通量应用
实验示例
图1.检测线粒体紊乱的灵敏度是jc-1的10倍。使用mito-id®膜电位染料（红色）或jc-1（蓝色）在经cccp处理的hela细胞中评估线粒体膜电位（mmp）。使用传统的荧光酶标仪检测，结果显示mmp随着cccp浓度的增加而减少，橙色荧光减少。染料的水溶性优化和无需洗涤的实验方案最大限度减小了可变性，因此z因子（>0.9）高于使用jc-1.
图2.在药物筛选中实时检测有丝分裂毒性。使用biotek synergy™ mx荧光酶标仪对线粒体膜电位变化的时间进程研究。hela细胞与mito-id®膜电位染料在室温下孵育30分钟（不去除血清或培养基）。添加鱼藤酮分别达到1µm、3µm和9µm的浓度。橙色信号的减少证明染料对鱼藤酮有反应。
产品信息
产品货号
enz-51019-kp002
产品名称
mito-id®membrane potential cytotoxicity kit
规格
1 kit
短期保存
-20°c
长期保存
-80°c
试剂盒组分
mito-id®mp detection reagent, 200 μl
cccp control, 100 μl
10x assay buffer 1: 2.5 ml
50x assay buffer 2: 0.5 ml
应用
htsmicroplate
部分产品引用文献
1. novel silver complexes based on phosphanes and ester derivatives of bis(pyrazol-1-yl)acetate ligands targeting trxr: new promising chemotherapeutic tools relevant to sclc management: m. pellei, et al.; int. j. mol. sci.24, 4091 (2023)
2. fundc1 regulates receptor-mediated mitophagy independently of the pink1/parkin-dependent pathway in rotenone-treated sh-sy5y cells: s.y. park, et al.; food chem. toxicol.137, 111163 (2020),application(s):fluorescence microscopy and microplate reader
3. inhibitory role of trip-br1/xiap in necroptosis under nutrient/serum starvation: z. sandag, et al.; mol. cells43, 236 (2020)
4. nad hydrolysis by the tuberculosis necrotizing toxin induces lethal oxidative stress in macrophages: d. pajuelo, et al.; cell. microbiol.22, e13115 (2020),application(s):thp-1 macrophages; microplate reader
5. impaired autophagic and mitochondrial functions are partially restored by ert in gaucher and fabry diseases: m.m. ivanova, et al.; plos one14, e0210617 (2019),application(s):fluorescence microscopy
6. inhibitory role of ampactivated protein kinase in necroptosis of hct116 colon cancer cells with p53 null mutation under nutrient starvation: d.t. le, et al.; int. j. oncol.54, 702 (2019)
7. ros as a novel indicator to predict anticancer drug efficacy: t. zaidieh, et al.; bmc cancer19, 1224 (2019)
8. trem1/3 deficiency impairs tissue repair after acute kidney injury and mitochondrial metabolic flexibility in tubular epithelial cells: a. tammaro, et al.; front. immunol.10, 1469 (2019),application(s):microplate reader
9. anti-cancerous effect of cis-khellactone from angelica amurensis through the induction of three programmed cell deaths: s. jung, et al.; oncotarget9, 16744 (2018),application(s):microplate reader
10. blue light phototoxicity toward human corneal and conjunctival epithelial cells in basal and hyperosmolar conditions: v. marek, et al.; free radic. biol. med.126, 27 (2018),application(s):microplate reader
11. cgas drives noncanonical-inflammasome activation in age-related macular degeneration: n. kerur, et al.; nat. med.24, 50 (2018)
12. cytotoxicity of propofol in human induced pluripotent stem cell-derived cardiomyocytes: k. kido, et al.; j. anesth.32, 120 (2018),application(s):microplate reader
13. development of novel amino-quinoline-5, 8-dione derivatives as nad (p) h: quinone oxidoreductase 1 (nqo1) inhibitors with potent antiproliferative activities: y. ling, et al.; eur. j. med. chem.154, 199 (2018)
14. inhibition of apoptosis using exosomes in chinese hamster ovary cell culture: s. han, et al.; biotechnol. bioeng.115, 1331 (2018)
15. light action spectrum on oxidative stress and mitochondrial damage in a2e-loaded retinal pigment epithelium cells: m. marie, et al.; cell death disc.9, 287 (2018)
详情请联系enzo life sciences金牌代理——欣博盛生物
发货美国FBA头程，如何选择货代公司？
Q41F软密封球阀机械部标准与化工部标准球阀差异对比分析
机床防护罩保护机床相关知识
抢占亚马逊购物车需要具备哪些条件？
澳大利亚专线物流公司 货运专线物流平台
线粒体膜电位细胞毒性检测试剂盒--ENZO LIFE SCIENCE热销产品
二手对辊式干法挤压造粒机转让
寄国际快递到德国多少钱一公斤
木工开料机是否会逐渐代替雕刻机？两者区别是什么
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箱式真空气氛炉常见故障及应对措施
MSC推出远东—埃弗格雷斯港直航服务
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凉水塔防腐专业施工经验丰富
20N数显式测力仪,2KG手持测力仪,20拉压测力传感器
国际空运承运人所造成的责任的划分是什么？
阻燃型玻璃鳞片防腐胶泥树脂型号
沟槽阀门
无线监控系统无线网桥的主从之分
兴港投资集团是国企吗（郑州航空港兴港投资集团有限公司简介）