Epicypher：BRD4 CUTANA™ CUT&RUN Antibody
epicypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。epicypher推出的cut&run级别的brd4 antibody符合epicypher的“cutana compatible”标准，可用于核酸酶靶向切割和释放（cut&run）和/或靶向剪切及转座酶技术（cut&tag）的基因组定位。brd4抗体主要在转录起始位点的侧翼产生cut&run峰(fig. 1)。正如文献所报道，brd4峰与brg1/smarca4峰有很大程度的重叠(fig. 2)。
产品详情
反应种属:human, mouse
宿主来源:rabbit
实验应用:cut&run, ihc, ip, wb
免疫原:between amino acids 1312 and 1362
克隆性:polyclonal
保存温度:stable for 1 year at 4°c from date of receipt
运输温度:do not freeze!! frozen cold packs.
产品形式:antigen affinity-purified antibody in tris-citrate/phosphate buffer ph 7-8, 0.09% sodium azide
验证数据
figure 1: brd4 peaks in cut&run
cut&run was performed as described above. peaks were called with macs2. heatmaps show brd4 peaks relative to igg negative control antibody and h3k4me3 positive control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. all rows were aligned relative to h3k4me3 antibody.
figure 2: brd4 cut&run representative browser tracks
cut&run was performed as described above. gene browser shots were generated using the integrative genomics viewer (igv, broad institute). three representative loci show overlap of brd4 and brg1 peaks.
figure 3: immunohistochemistry data
ffpe sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) using brd4 antibody at a dilution of 1:5,000.
figure 4: immunoprecipitation data
epicypher brd4 antibody (6 µg) was used to immunoprecipitate whole cell lysates (1 mg, 20% of ip loaded) isolated from hela cells. a negative control igg antibody and positive control antibody targeting brd4 (bethyl laboratories) were also used to demonstrate the specificity of the ip. for blotting immunoprecipitates, bethyl laboratories brd4 antibody (a700-004) was used at a dilution of 1:1,000.
订购详情
货号
产品名称
规格
13-2003
brd4 cutana™ cut&run antibody
50 µl
如需了解更多详细信息或相关产品，请联系epicypher中国授权代理商-欣博盛生物
CD Automation功率控制器RE3450-42VPUV3024
树脂粉微波干燥设备的特点有哪些？
Shopee卖家寻找货源的渠道有哪些,shopee无货源怎么让卖家发货
美国空运斯塔特大学机场介绍
海康大华巨头当前 AI安防新手商汤该如何立足？
Epicypher：BRD4 CUTANA™ CUT&RUN Antibody
直高发行业标准中直流高压发生器试验方法
你知道什么是土壤标准物质么？看看本篇吧
国际空运的运作方式有哪些（国际空运干货知识分享）
FYD型多级液下泵产品用途和结构
南京理工*JH20-1C
应用VOCs废气处理技术时应该如何避免“踩坑”
UPS 国际快递——高效、可靠的全球物流服务
【国际海运动态】船公司APL退出商业运营服务美国政府！
脱碳谈判并未阻止燃油价格上涨
数控机床电气控制故障与保修策略研究
韩国化妆品网站（韩国化妆品正品网站)
ST5541测试仪 9784单元 日本日置HIOKI
香港直邮巴中-巴中快递到香港
实验室日常安全用电
产品详情
反应种属:human, mouse
宿主来源:rabbit
实验应用:cut&run, ihc, ip, wb
免疫原:between amino acids 1312 and 1362
克隆性:polyclonal
保存温度:stable for 1 year at 4°c from date of receipt
运输温度:do not freeze!! frozen cold packs.
产品形式:antigen affinity-purified antibody in tris-citrate/phosphate buffer ph 7-8, 0.09% sodium azide
验证数据
figure 1: brd4 peaks in cut&run
cut&run was performed as described above. peaks were called with macs2. heatmaps show brd4 peaks relative to igg negative control antibody and h3k4me3 positive control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. all rows were aligned relative to h3k4me3 antibody.
figure 2: brd4 cut&run representative browser tracks
cut&run was performed as described above. gene browser shots were generated using the integrative genomics viewer (igv, broad institute). three representative loci show overlap of brd4 and brg1 peaks.
figure 3: immunohistochemistry data
ffpe sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) using brd4 antibody at a dilution of 1:5,000.
figure 4: immunoprecipitation data
epicypher brd4 antibody (6 µg) was used to immunoprecipitate whole cell lysates (1 mg, 20% of ip loaded) isolated from hela cells. a negative control igg antibody and positive control antibody targeting brd4 (bethyl laboratories) were also used to demonstrate the specificity of the ip. for blotting immunoprecipitates, bethyl laboratories brd4 antibody (a700-004) was used at a dilution of 1:1,000.
订购详情
货号
产品名称
规格
13-2003
brd4 cutana™ cut&run antibody
50 µl
如需了解更多详细信息或相关产品，请联系epicypher中国授权代理商-欣博盛生物
CD Automation功率控制器RE3450-42VPUV3024
树脂粉微波干燥设备的特点有哪些？
Shopee卖家寻找货源的渠道有哪些,shopee无货源怎么让卖家发货
美国空运斯塔特大学机场介绍
海康大华巨头当前 AI安防新手商汤该如何立足？
Epicypher：BRD4 CUTANA™ CUT&RUN Antibody
直高发行业标准中直流高压发生器试验方法
你知道什么是土壤标准物质么？看看本篇吧
国际空运的运作方式有哪些（国际空运干货知识分享）
FYD型多级液下泵产品用途和结构
南京理工*JH20-1C
应用VOCs废气处理技术时应该如何避免“踩坑”
UPS 国际快递——高效、可靠的全球物流服务
【国际海运动态】船公司APL退出商业运营服务美国政府！
脱碳谈判并未阻止燃油价格上涨
数控机床电气控制故障与保修策略研究
韩国化妆品网站（韩国化妆品正品网站)
ST5541测试仪 9784单元 日本日置HIOKI
香港直邮巴中-巴中快递到香港
实验室日常安全用电